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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Neuroprotective Effect of Taohong Siwu Decoction on Cerebral Ischemia/Reperfusion Injury via Mitophagy-NLRP3 Inflammasome Pathway
doi: 10.3389/fphar.2022.910217
Figure Lengend Snippet: THSWD treatment inhibits the generationthe of ROS, activation of NLRP3 inflammasome and secretion of pro-inflammatory cytokine. (A) Quantification of ROS levels; n = 6 per group. (B – I) The Western blot relative band density of NLRP3, ASC, cleaved-caspase-1, IL-1β, IL-18; n = 6 per group. Each experiment was repeated 3 times. ** p < 0.01, *** p < 0.001 versus sham group, # p < 0.05, ## p < 0.01, ### p < 0.001 versus MCAO/R group, & p < 0.05, && p < 0.01, &&& p < 0.001 versus MCAO/R + THWSD group.
Article Snippet: Blocked with 5% nonfat milk, the membranes were incubated with the appropriate primary antibodies NLRP3 (ab263899, 1:1000, Abcam), PINK1 (af7755, 1:1000, Beyotime, China), LC3B (ab192890, 1:2000 Abcam), Parkin (ABP55480, 1:1000, abbkine, China), caspase-1 (ab179515, 1:1000, Abcam),
Techniques: Activation Assay, Western Blot